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KMID : 0351619730140020255
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Kyungpook Medical Journal
1973 Volume.14 No. 2 p.255 ~ p.266
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Effects of Reduced Glutathione and Ginseng Extract on Non-Protein Sulfhydryl and Non-Protein Disulfide of the Mouse Liver and Blood Following Fractionated Whole Body X-Irradiation
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Abstract
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Of the sulfhydryl substances which have been studied for the possible radioprotective actions when injected into the animal prior to ionizing radiation, reduced glutathione (GSH) has been proved to be the most effective.
Lately. Oh reported that the extract from Panax Ginseng contains some amount of sulfhydryl group and suggested the applicability of the extract as radioprotector.
In the present study, an effort was directed to further clarify the radloprotective action of GSH, and to observe a possible radioprotective action of Ginseng extract.
The mouse was subjected to whole body X-irradiation in fractionated daily dose of 300R for four days, either independently or immediately following the injection of 1㎖ of GSH containing 1㎎ CSH per gm body weight, or Ginseng extract containing 2.4μmol of sulfhydryl suastance, to the mouse intraperitoneally.
The experiment was divided into two parts. In the first experiment, the time of observailon was set at 30, 60 and 120 min, and in the second experiment, the time interval was further divided as 5, 10, 20, and 30 min in order to observe radioprotective actions of GSH and Ginseng extract within the relatively shorter period.
The non-protein sulfhydryl (NP-SH) and nonprotein disulfide (NP-SS) levels of the lever, and NP-SH level of the blood of the mouse were measured in the first and second experiments. and the results were compared with the normal values.
The results thus obtained are summarized as follows.
1) The normal values of NP-SH and NP-SS of the mouse liver were 5.90± 0.46/μmol/gm wet., and 3.02±0.42/μmol/gm wet wt., respectively, and the normal value of NP-SH of the mouse blood was 3.98±1.29/μmol/ml.
2) The higllest liver NP-SH was observed at 60 min in fractionated X-irradiation group and GSH injected group, but in Ginseng injected group, the liver NP-SH was similar to the control value throughouth the experiment.
When GSH and fractionated X-irradiation were combined, the liver NP-SH was the highest at 60 min, but when Ginseng extract was injected perior to fractionated X-irradiation, the highest liver NP-SH was observed at 30 min.
3) When the change of the liver NP-SH was observed within 30 min, a tendency of the highest value at 5 min followed by the decrease at 30 min was observed, and the tendency was most prominent when Ginseng extract and fractionated X-irradiation were combined.
4) The liver NP-SS showed the highest values at 30 min in most groups, but when GSH was combined with fractionated X-irradiation, higher liver NP-SS was observed at the latter part of the experiment.
5) Within 30 min of the experiment, the liver NP-SS showed a general tendency of the highest values at 5 min in most groups, with the exception of GSH injected group and combined group of Ginseng extract and fractionated X-irradiation, where the liver NP-SS were elevated till 30 min.
6) The blood NP-SS were elevated at 30 min ia GSH and Ginseng extract injected groups, but in fractionated f-irradiation, the blood NP-SH were highest at 60 min before decreasing to the control value at 120 min.
7) The blood NP-SH within 30 min of the experiment showed a general tendency of the elevated value at 5 min followed by a. decrease at 30 min, but in the group where Ginseng extract and fractionated X-irradiation were combined, the blood NP-SH did not change from the control till 30 min.
8) From the above results, the radioprotective action of GSH is demonstrated throughout the experiment, but the radioprotective action of the Ginseng extract seems to be limited only within 30 min of the injection.
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